The anti-tank ammunition was a basic Armor-Piercing (AP) shot, but by January 1943 an Armor-Piercing, Capped (APC) shot and an Armor-Piercing, Capped, Ballistic Capped (APCBC) shot was supplied. Then by 1944, the Armor-Piercing, Composite Rigid (APCR) shot, and the Armor-Piercing, Discarding Sabot (APDS) shot were supplied. There was a considerable difference in effectiveness, as the APC shot could penetrate up to 92mm of armor, but the APDS could penetrate armor up to 142mm thick. Furthermore, a high explosive shell was produced so that the gun could be used against un-armored targets as well.
The 6-pounder first saw action in May 1942 in Northern Africa. At the Second Battle of El Alamein the 6-pounder guns of 2nd Battalion, together with the 239 Anti-Tank Battery Royal Artillery, destroyed more than 15 enemy tanks. As the Germans produced much heavier armored tanks, the Tiger I and Panther, the standard 6-pounder shot proved to be ineffective against their front armor. However, they still proved effective on the less armored sides and rear armor. The ineffectiveness of the 6 pounder against the heavily armored Tigers was somewhat improved by the development of more powerful ammunition, in the form of the Armor-Piercing, Composite Rigid (APCR) shot, and the Armor-Piercing, Discarding Sabot (APDS) shot, which was available from 1944 and made it effective in fighting the Tiger I and Panther tanks frontally.